Вестник МГТУ, 2024, Т. 27, № 3.

Вестник МГТУ. 2024. Т. 27, № 3. С. 294-301. DOI: https://doi.org/10.21443/1560-9278-2024-27-3-294-301 blood plasma (according to GOST 31746-2012 "Food products. Methods for detecting and quantifying coagulase-positive staphylococci and Staphylococcus aureus "). Colonies of microorganisms retain their morphological and cultural properties. Therefore, for these test cultures, nutrients were sufficient on all studied media. The results of microbiological studies confirm the possibility of using fish protein hydrolysate obtained in the process of hydrolysis from cod musculoskeletal waste as a component of general-purpose nutrient media for counting colonies of microorganisms. Conclusion Thus, as a result of the conducted research: - the parameters of the fermentolysis are optimized: duration - 3 hours, enzyme concentration - 1.33 %; - there are determined chemical and biochemical parameters of the prototype fish protein hydrolysate (mass fraction of amine nitrogen must be at least 3.6 %, total nitrogen - at least 13.0 %), which meet the requirements for the indicators of protein components of nutrient media; - the quality of nutrient media using a prototype fish protein hydrolysate as a protein component to the operational criteria of GOST ISO 11133-2016 is established; - the developed fish protein hydrolysate can be recommended for the preparation of general-purpose culture media for counting colonies of microorganisms. It has been also determined that in the culture medium "Nutrient agar for the cultivation of microorganisms dry GRM" the developed product could completely replace "Pancreatic hydrolysate of fish meal". Conflict of interest The authors declare no conflict of interest. Acknowledgments The study was carried out through proactive R&D of Murmansk Arctic University "Development and improvement of technologies for the production of food, feed and technical products of a wide range from animal and plant raw materials of the Arctic region". References Derkach, S. R., Grokhovsky, V. A., Kuranova, L. K., Volchenko, V. I. 2017. Nutrient analysis of underutilized fish species for the production of protein food. Foods and Raw Materials, 5(2), pp. 15-23. DOI: https://doi.org/10.21603/2308-4057-2017-2-15-23. EDN: YQZBYK. Dvoryaninova, O. P., Sokolov, A. V. 2015б. Secondary products of fish cutting: Sources, properties and application in the production of natural biopolymers. Proceedings of V Intern. conf. Innovative food technologies in the storage and processing o f agricultural raw materials: fundamental and applied aspects , Krasnodar, 28-29 May, 2015. Voronezh, pp. 186-190. (In Russ.) Dvoryaninova, O. P., Sokolov, A. V., Spiridonova, M. V. 2015а. By-products of fish cutting: Composition, properties and application. Sovremennyye Tendentsii Razvitiya Nauki i Tekhnologiy, 5-2, pp. 17-21. EDN: UHWLQX. (In Russ.) Kasyanov, G. I. 2015. Modern breakthrough technologies in the meat, dairy and fish industries. Proceedings of Intern. conf. Innovative technologies for processing raw materials o f animal origin, Krasnodar, 20 February, 2015. Krasnodar, pp. 8-11. EDN: UXUJBV. (In Russ.) Kuranova, L. K., Zhivlyantseva, I. V. 2016. Study of the biological value of peptone obtained from secondary fish raw materials of the Arctic region. Coll. of articles of All-Russ. scientific-practical conf. with international participation dedicated to the 145th anniversary of the Sevastopol Biological Station Marine biological research: Achievements and prospects, 19-24 September, 2016. Sevastopol. In 3 vol., 3 Vol., pp. 401-403. EDN: XEVIZN. (In Russ.) Lomovtseva, A. V., Kulikova, T. V. 2019. Development of the fishery complex in the context of import substitution. Proceedings of the national scientific and technical conf. Scientific and practical issues o f fisheries regulation, Vladivostok, 22-23 May, 2019. Vladivostok, pp. 74-79. EDN: KZVCHJ. (In Russ.) Maksimyuk, N. N., Maryanovskaya, Yu. V. 2009. On the advantages of the enzymatic method for obtaining protein hydrolysates. Fundamental Research, 1, pp. 34-35. EDN: KVFGDT. (In Russ.) Production and use of microbiological products: A review information. 1990. Inform. provision of industry scientific and technical programs and main directions of industry development. Moscow. (In Russ.) Reshetnikov, M. T. 2000. Experiment planning and statistical data processing. Tomsk. (In Russ.) Zhivlyantseva, I. V., Kuranova, L. K., Volchenko, V. I., Grokhovsky, V. A. 2018. Peptone from by-products of Atlantic cod processing: Technology, quality, use. Bulletin o f Kamchatka State Technical University, 45, pp. 28-36. DOI: https://doi.org/10.17217/2079-0333-2018-45-28-36. EDN: YAAMBN. (In Russ.) 299