Physics of auroral phenomena : proceedings of the 35th Annual seminar, Apatity, 28 Februaru – 02 March, 2012 / [ed. board: A. G. Yahnin, A. A. Mochalov]. - Апатиты : Издательство Кольского научного центра РАН, 2012. - 187 с. : ил., табл.

Cytogenotoxic effects o fexposure to ionizing radiation in the miners buccal cells radiation, include the substantial decrease of the normal cells, the increase of the number of cells with necrotic changes and the increase more than an order of the binucleated cells in compare with the control group. The sharp increase of the binucleate cells in the buccal epithelium of examinees from exposed group in compare with the unexposed group could indicate the influence of the ionizing radiation on the process of cytokinesis. The studies on the cell cultures have shown that the number of multinucleate cells in cell cultures of different ontogenetic and phylogenetic origin increased synchronously with the increasing intensity of the neutron component of the Earth's surface by solar proton events (Belisheva et al., 2012). It is possible that the neutron component contribution when miners irradiating by mixed sources of ionizing radiation can be manifested in the increase of the multinucleate cells frequency in the miners’ buccal epithelium. The increase of binucleate cells frequency in 2.26 times in the miners’ buccal epithelium and apoptosis reducing in 1.8 times in compared with the unexposed group indicates the low efficiency of the apoptosis of the miners designed to eliminate the defective genetic material. This is an unfavorable prognostic sign and indicates a high degree genotoxicity of mixed types of ionizing radiation in mining production. Acknowledgments. This work was supported by the Russian Foundation for Basic Research (RFBR) and Murmansk government, project № 10-04-98809-р_ север_а. References Мейер A.B.; Дружинин В.Г., Ларионов А.В., Толочко В.А. Генотоксические и цитотоксические эффекты в буккальных эпителиоцитах детей, проживающих в экологически различающихся районах Кузбасса // Цитология. - 2010. - Т.52, №4. - С.305-310. Пелевина И.И., Афанасьев Г.Г., Алещенко А.В., Антощина М.М, Готлиб В.Я., Конрадов А.А., Кудряшова О.В., Лизунова Е.Ю., Осипов А.Н., Рябченко Н.И., Серебряный А.М. Молекулярно-клеточные последствия аварии на ЧАЭС // Радиац. биология. Радиоэкология. - 2011. - Т. 51, № 1. - С. 154-161. Петрашова Д.А., Белишева Н.К., Пелевина И.И., Мельник Н.А., Зользер Ф. Генотоксические эффекты в буккальном эпителии горняков, работающих в условиях облучения природными источниками ионизирующего излучения // Известия Самарского научного центра Российской академии наук. - 2011, Т. 13, №1(7).-С . 1792-1796. Belisheva N.K., Lammer Н., Biemat Н.К., Vashenyuk V.E. The effects of cosmic rays on biological systems - an investigation during GLE events // Astrophysics and Space Sciences Transactions, 2012, 8. - P. 7-17. Holland N., Bolognesi C., Kirsch-Volders М., Bonassi S., Zeiger E., Knasmueller S., Fenech M. The micronucleus assay in human buccal cells as a tool for biomonitoring DNA damage. The HUMN project perspective on current status and knowledge gaps // Mutat Res. - 2008. - Vol.659(l-2). - P.93-108. Stich H.F., San R.H., Rosin M.P. Adaptation of the DNA-repair and micronucleus tests to human cell suspensions and exfoliated cells // Ann. N.Y. Acad. Sci. - 1983. - Vol. 407. - P. 93-105. Tolbert P.E., Shy C.M., Allen J.W. Micronuclei and other nuclear anomalies in buccal smears: methods development// Mut. Res. - 1992. - 271. - P. 69-77. 185