Sandimirov S. Specific Features of Accumulation of Cu, Ni, Zn, Cd and Hg in Two Whitefish Coregonus lavaretus (L.). Morphs Inhabiting the Inari-Pasvik Lacustrine-Riverine System. Inland Water Biology. 2011, Vol. 4, №, p. 383-392.

384 KASHULIN et al. Table 1. Concentrations of heavy metals in water (p.g/l) and surface layers of BSs (p.g/g, mean values) of the studied water­ bodies in the Pasvik River basin [1, 22] Waterbody Cu Ni Zn Mn Sr Pb Cr Cd Hg In Water Kuetsjarvi 9.6 11(5 4.1 42 62 0.23 0.3 0.07 <0.01 6.3-18.- 74-182 1.3 - 19 5.1-260 32-94 0-1 .03 0.1-1. 1 0-0 .28 Skrykkebukta 2.4 9.0 5.1 3.6 20 0.27 0.3 0.05 <0.01 1.6-3.5 6.3- 13.8 0.6-0 . 28 1.4 - 6.9 17- 23 0-0. 73 0.1-0. 4 0-0 .09 Vaggetem 1.7 2.1 3.5 11.3 15 0.37 0.2 0.05 <0.01 0.7-5.4 0.3-15.2 0. 1-50 1.4-72 11 - 19 0-1 .10 0.1-0. 7 0-0 .22 Rajakoski 1.8 1.6 2.2 3.9 19 0.18 0.2 0.14 <0.01 0.4-4.3 0.8-2 . 5 0.5-4 . 9 2.4-5. 2 18- 20 0. 10-0. 28 0.2-0. 3 0.06-0 .26 Inari 0.7 1. 1 2.3 11.4 19 0.19 <0.2 0.10 <0.01 0.5-1.- 0.5-2 . 3 0.6-5. 8 1.6-58 17- 20 0.09-0 .34 0.05-0 .29 In the surface layers of BSs Kuetsjarvi 1215 2839 254 3630 64 38.7 206 2.47 0.16 Skrykkebukta 169 280 127 570 47 26.8 116 0.27 0.02 Vaggetem 71 79 109 380 35 16.4 103 0.13 0.10 Rajakoski 32 37 81 435 50 8.8 61 0.18 0.14 Inari 37 51 104 78425 49 23.7 51 0.47 0.09 Notes: mean is on the top; limits are on the bottom. sia Bordering Area.” All the studied waterbodies are subject to air technogenic pollution, the intensity of which exhibits a clear dependence on the distance from the source o f pollution. Lake Kuetsjarvi and Skrykkebukta Reservoir, which are situated in the river downstream, are subject to the impact o f wastewaters outflowing from the ore fields, smelters, and stock­ piled tailings o f the Pechenganickel smelter plant. Some hydrochemical parameters o f studied waterbod­ ies and indices o f the pollution o f BSs [22] character­ izing the levels o f technogenic load on the waterbodies are given in Table 1. The indices o f contamination of BSs were used to assess the levels of the loads [1, 4, 22]. The contents o f HMs in the whitefish organism were determined along the whole length o f the Pasvik River in the direction towards an increasing intensity of pol­ lution in the following order: lakes Inari—Rajakoski— Vaggetem—Skrykkebukta—Kuetsjarvi (Fig. 1). All val­ ues o f HM content in BSs and fish tissues are given as per dry weight. The HM contents were determined in two ecologi­ cal morphs ( “medium rakered” and “sparsely rak- ered”) o f common (European) whitefish, Coregonus lavaretus (Linnaeus, 1758), the dominant fish species in the basin, which occupies different ecological niches [9]. The fish were caught in the littoral, profun- dal, and pelagic zones o f the waterbodies using fixed bottom and floating gill nets [3, 22]. The nets were set one by one in the littoral zone perpendicular to the shoreline (10 net); in the profundal zone, they were set in one order o f up to ten nets. The floating nets were placed on the open parts o fwaterbodies isolating a 0-to 6-m water layer. The fish were analyzed shortly after sampling using the commonly accepted routine [3, 8]. To determine the HM contents in fish organs and tissues, 10— 15 specimens o f similar sizes were selected. The liver, kidney, gill, and pieces o f muscles were sampled. The organs and pieces o f tissues were dissected using a stainless steel lancet and knife placed in plastic bags and frozen for transportation to the laboratory and for storage. Chemical analyses were performed in the Institute o f the Industrial Ecology Problems o f the North, Kola Science Center, Russian Academy o f Sci­ ences. In the laboratory, the tissue sample was dried to a constant weight at 105°C. The samples were decom­ posed in a Multiwave 3000 microwave oven (Anton Paar, Austria) in Teflon autoclaves according to the program for the decomposition o f fish soft tissues given in the guidelines: 0.5—0.7 g tissue sample, 2 ml water, 4 ml NHO3, 0.5 ml HCl, 13 min at 600 W power, and 10 min at 400 W, followed by chilling for 20 min. After the sample decomposed, its volume was adjusted to 15 ml. The contents of Cu, N i and Zn were deter­ mined using PerkinElmer 5000 atomic absorption spec­ trophotometer with an HGA-400 graphite atomizer after the initial samples were dissolved 5—50 times, depending on the sample. The content o f Hg was INLAND WATER BIOLOGY Vol. 4 No. 3 2011