Sandimirov S. Screening studies of POP levels in fish from selected lakes in the Paz watercourse / In State of the environment in the Norwegian, Finnish and Russian border area. The Finnish Environment. Finland, Jyvaskyla: Kopijyva Oy. 2007, №6.

Screening studies o f POPs in fish from the Paz watercourse For biochemical (biomarker) analyses, liver and fish bile were collected at two locations (Kuetsjarvi and Rajakoski). To quantify bile acids in fish bile, gallbladders were dissected out and bile was extracted into glass flasks and fixed by 96% ethanol (volume per cent). Samples were stored at 4oC until analysis. To assess of aniline hydroxylase activity in fish liver microsomes, hepatic tissues from each fish were removed in package and frozen on liquid nitrogen. Samples were stored at liquid nitrogen until analysis. Liver and muscle samples from fish of the same age and sex were pooled and analysed for a wide range of POPs and mercury. We expect that there is limited variance between the fish in the pooled samples. 4 Analyses 4.1. Analysed material Due to limited funding for analyses, two species, whitefish and pike from selected localities (Kuestjarvi, Ruskebukta, Skrukkebukta, Tj^rebukta and Stuorajavri), were selected for contaminant analyses. Other fish species are stored in a freezer and will be available for future analyses. The analysed material and the main parameters are given in Table 2. The complete results are given in Appendix 3. Table2. Concentrations o f LDDT, Hexachlorobenzene (HCB), Chlordanes (ECHL), Hexachlorcyclohexane (EHCH), EPCB, Toxaphene, LPCDD/F, EPBDE and LPAH in samples from fish from Tjwrebukta, Skrukkebukta, Ruskebukta, Kuetsjarvi and Lake Stuorajavri (lower table). All concentrations are given in ng/g ww exceptfor LPBDE (pg/g ww). Component White fish Pike Tjsrebukta Skrukkebukta Ruskebukta Kuetsjarvi Kuetsjarvi 4-5 years, 3-5 years, 4-6 years, 3-5 years, Wt 100-500 g Wt 100-300 g Wt 300-500 g Wt 50-140 g Muscle Liver Muscle Liver Muscle Liver Muscle Liver Muscle Liver N=10 N=10 N=5 N=5 N=5 N=5 N=10 N=15 N=10 N=10 Lipids, % 0.66 14.1 0.88 5.25 0.54 7.94 1.86 6.99 0.41 25.9 1EDDT 3.59 1.96 0.32 3.47 n.d. 1.66 17.5 88.1 3.59 109 Hexachlorobenzene (HCB) n.d. 1.23 0.28 0.69 0.15 0.97 0.58 2.81 0.20 3.71 2Chlordanes (ECHL) 0.17 0.30 n.d. n.d. n.d. n.d. 0.18 0.46 0.05 3.00 3Hexachlorcyclohexane (EHCH) n.d. n.d. 0.07 n.d. n.d. n.d. 0.29 1.51 n.d. 5.50 4EPCB 5Toxaphene 4.94 3.24 5.1 30.7 0.94 n.d. 8.32 n.d. 0.93 n.d. 3.79 5.73 21 6.27 150 n.d. 5.84 3.24 283 138.9 EPCDD/F n.d. 2.52 n.d. n.d. n.d. n.d. n.d. n.d. n.d. 7.39 6EPBDE 1.72 39.4 11.9 75.5 0.50 30.3 137 758 29.4 2968 7EPAH n.d. 34.1 7.86 101.8 6.26 73.6 2.48 286 n.d. 155.5 Akvaplan-niva report APN 514-3365.02 7