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Appendix: Screening studies o f POPs in fish from the Paz watercourse Instrumental analysis (HRGC/LRMS) Polychlorinated biphenyls and OCPs The analysis was performed with GC/MS Varian Saturn 2200 T. Calibration of the instrument was carried out using a standard mixture of biphenyls BP-MS, Wellington Laboratories and SRM-1492, NIST. Results of analyses were processed with software package Varian 5.2. Toxaphenes, Polychlorinated dibenzo-p-dioxins/dibenzofurans and Polybrominated diphenyl ethers The analysis was performed with GC/MS Varian Saturn 1200 using chemical ionization with detection of negative ions (NCI) in the selective ion monitoring (SIM) mode. The reagent gas was methane. Calibration of the instrument was carried out using standard solutions of Toxaphenes TOX 482, PROMOCHEM, standard solutions of PCDD/PCDF prepared on the base of the mixture EDF 7999 Cambridge Isotope Lab.and standard solutions of PBDE prepared on the base of the mixture EO- 4980, Cambridge Isotope Lab. Analysis of trace elements The trace element analyses were carried out by AAS method after decomposition of the bottom sediment samples with nitric acid. Cd, Ni and Pb were measured by a furnace technique on Perkin Elmer Z-3030 with Zeeman correction of background. Zn and Cu were measured by AAS method by flame procedure on Perkin Elmer B-3030. Hg was measured in bottom sediment and fish samples by method of “cold vapor” on MHS 15 after a decomposition of a sample by mixture of sulphuric and nitric acids. QA/QC results The internal QA/QC program in samples analysis for organic pollutants involved control for possible contamination of samples during sample preparation and measurements. The analysis was performed in batches. Each batch included a procedural blank, spiked blank sample with the known content of added analytes. Recovery of analytes was controlled using isotope-labelled analogues of determined analytes. QA/QC results are presented in the Tables 1.1 - 1.14. Quality assurance and quality control for heavy metals included analyses of blanks, analyses of duplicates, use of reference materials and matrix spike recoveries. Results on QA/QC analysis for biota samples are presented in table 1.15-1.16. Akvaplan-niva report APN 514-3365.02 37